باید کد بنویسید 

private void InsertData(string connectionString, string firstName, string lastname, string username, string password
                        int Age, string gender, string contact)
{
    // define INSERT query with parameters
    string query = "INSERT INTO dbo.regist (FirstName, Lastname, Username, Password, Age, Gender,Contact) " + 
                   "VALUES (@FirstName, @Lastname, @Username, @Password, @Age, @Gender, @Contact) ";

    // create connection and command
    using(SqlConnection cn = new SqlConnection(connectionString))
    using(SqlCommand cmd = new SqlCommand(query, cn))
    {
        // define parameters and their values
        cmd.Parameters.Add("@FirstName", SqlDbType.VarChar, 50).Value = firstName;
        cmd.Parameters.Add("@Lastname", SqlDbType.VarChar, 50).Value = lastName;
        cmd.Parameters.Add("@Username", SqlDbType.VarChar, 50).Value = userName;
        cmd.Parameters.Add("@Password", SqlDbType.VarChar, 50).Value = password;
        cmd.Parameters.Add("@Age", SqlDbType.Int).Value = age;
        cmd.Parameters.Add("@Gender", SqlDbType.VarChar, 50).Value = gender;
        cmd.Parameters.Add("@Contact", SqlDbType.VarChar, 50).Value = contact;

        // open connection, execute INSERT, close connection
        cn.Open();
        cmd.ExecuteNonQuery();
        cn.Close();
    }
}

protected void Button1_Click(object sender, EventArgs e)
   {
       DataTable dt = new DataTable();

       if (dt.Columns.Count == 0)
       {
           dt.Columns.Add("PayScale", typeof(string));
           dt.Columns.Add("IncrementAmt", typeof(string));
           dt.Columns.Add("Period", typeof(string));
       }

       DataRow NewRow = dt.NewRow();
       NewRow[0] = TextBox1.Text;
       NewRow[1] = TextBox2.Text;
       dt.Rows.Add(NewRow); 
       GridView1.DataSource = dt;
       GridViewl.DataBind();
   }

کمی خلاقیت به خرج بدید و کد رو اصلاح کنید تا نیازتون برطرف بشه